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Efficacy of Autologous Bone Marrow–Derived Stem Cell Transplantation in Patients With Type 2 Diabetes Mellitus


Progressive and inexorable β-cell dysfunction is the hallmark of type 2 diabetes mellitus (T2DM) and β-cell regeneration using stem cell therapy may prove to be an effective modality. A total of 10 patients (8 men) with T2DM for >5 years, failure of triple oral antidiabetic drugs, currently on insulin (≥0.7 U/kg/day) at least for 1 year, and glutamic acid decarboxylase antibody negative were included. Patients on stable doses of medications for past 3 months were…

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Intramyocardial autologous bone marrow cell transplantation for ischemic heart disease: A systematic review and meta-analysis of randomized controlled trials


Objective This study was undertaken to evaluate the efficacy of intramyocardial bone marrow cell (BMC) transplant therapy for ischemic heart disease (IHD). Methods The PubMed, Embase, and Cochrane Library databases through October 2013 were searched for randomized clinical trials (RCTs) of intramyocardial BMCs to treat IHD. The primary endpoint was change in left ventricular ejection fraction (LVEF). Secondary endpoints were changes in left ventricular end-systolic volume (LVESV) and left ventricular end-diastolic volume (LVEDV). Weighted mean…

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Abstract 140: Effect of Transendocardial Autologous Cardiac Stem Cells and Bone Marrow Mesenchymal Stem Cells to Reduce Infarct Size and Restore Cardiac Function in a Heart Failure Swine Model


Background: A cell combination of human mesenchymal stem cells (MSCs) and c-kit+ cardiac stem cells (CSCs) improves left ventricular (LV) performance to a greater degree than MSCs alone in post myocardial infarction swine. To advance the development of cell combination therapy, we administered autologous swine cells, and tested the hypothesis that transendocardial autologous CSCs/MSCs produces greater improvement of performance than MSCs in a rigorous model of heart failure due to post infarct LV remodeling. Methods:…

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Human mesenchymal stem cells as a tool for joint repair in rheumatoid arthritis


Rheumatoid arthritis (RA) is char – acterised with chronic inflammatory synovitis and progressive joint. Be – cause damaged and/or deformed joints cannot be repaired, a novel treatment strategy aimed at both anti-inflamma – tion and bone regeneration is a prereq – uisite. Mesenchymal stem cells (MSCs) can be easily isolated from various or – gans and possess multipotent capacity and exhibit immunoregulatory proper – ties. Using human MSC derived from bone marrow and adipose tissue,…

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Arterial regeneration and clinical improvement of secondary autoimmune Raynaud’s syndrome with autologous bone marrow mononuclear cells (aBM-MNC). early results of phase II clinical trial


Raynaud phenomenon is chronic vasospasm of the fingers, ears, nose and toes that occurs in response to stress or cold exposure, with a prevalence rates of 11% in women and 8% in men according with EEUU statistics. Secondary Raynaud phenomenon is associated with another disease like autoimmune disease. In our clinical center since 2010, 22 patients with secondary severe Raynaud syndrome (Lupus, scleroderma-CREST, vasculitis, Rheumatoid Arthritis) were recruited to the program of autologous cellular therapies…

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Homing of mesenchymal stem cells: mechanistic or stochastic? Implications for targeted delivery in arthritis


Mesenchymal stem cells (MSCs) are multipotent cells with the capacity to undergo chondrogenic differentiation. Systemically administered MSCs have been shown to preferentially accumulate at sites of tissue damage and inflammation, thus MSC-based therapy holds great promise for the treatment of inflammatory diseases such as RA. Modulation of MSC homing may allow targeted delivery of systemically administered MSCs to damaged articular cartilage, where they can suppress immune-mediated cartilage destruction and contribute to cartilage repair via a…

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AB0095 By intravenous infusion marked by green fluorescent protein to reveal bone marrow mesenchymal stem cells’ distribution and differentiation of collagen induced arthritis rats


Background Rheumatoid arthritis (RA) is a autoimmune disease,which is characterized by the osteoclasia or the high deformity rate of cartilage and bone. According to some materials, Mesenchymal stem cells (MSCs) were definited as the cell full of proliferation, differentiation capacity, and potential immune regulation. MSCs transplantation could be a appropriate-designed pattern to the joint damaging from rheumatoid arthritis (RA).However, the repairing mechanism against osteoclasia of cartilage and bone is still unclear. Objectives This study used…

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Mesenchymal Stem Cell Alterations in Bone Marrow Lesions in Patients With Hip Osteoarthritis


In patients with osteoarthritis (OA), bone marrow lesions (BMLs) are intimately linked to disease progression. We hypothesized that aberrant multipotential stromal cell (also known as mesenchymal stem cell [MSC]) responses within bone tissue contributes to BML pathophysiology. The aim of this study was to investigate BML and non-BML native subchondral bone MSCs for numeric, topographic, in vitro functional, and gene expression differences. Ex vivo 3T magnetic resonance imaging (MRI) of the femoral heads of 20…

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Impact of Autologous Bone Marrow-Derived Stem Cells on Degenerative Changes of Articulating Surfaces Associated With the Arthritic Temporomandibular Joint: An Experimental Study in Rabbits


This study evaluated the effect of bone marrow-derived stem cells (BMSCs) on degenerative changes of TMJ associated with bovine collagen type II (BCII) induced-arthritis. Materials and Methods Fifty rabbits were divided into 3 groups. Group I (n = 10) received no treatment. Group II (n = 20) was divided into 2 subgroups according to treatment type. One subgroup (n = 10) received an intra-articular (IA) injection of phosphate buffered saline (PBS) and the other (n = 10) received an IA injection of PBS…

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Bone marrow-derived mesenchymal stem cells inhibit T follicular helper cell in lupus-prone mice


The objective of this paper is to analyze the role of bone marrow-derived mesenchymal stem cells (BM-MSCs) on the differentiation of T follicular helper (Tfh) cells in lupus-prone mice. Bone marrow cells were isolated from C57BL/6 (B6) mice and cultured in vitro, and surface markers were identified by flow cytometry. Naïve CD4+ T cells, splenocytes and Tfh cells were isolated from B6 mice spleens and co-cultured with BM-MSCs. The proliferation and the differentiation of CD4+…

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