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Mushroom extract increases p53 expression and causes cell cycle arrest and apoptosis in a breast cancer cell line. *

Mushrooms are part of the sexual life cycle of particular fungi with specific metabolic pathways, and therefore may contain a largely unexploited source of powerful new pharmaceutical products with potential antitumor properties [1,2]. Furthermore, they may have potential as functional foods. Suillus collinitus is an edible mushroom found in European pine forests. The aim of this work was to study the cytotoxic potential of extracts from this mushroom in various cancer cell lines. Different extracts (methanolic, ethanolic and aqueous) were prepared and extractinduced cell growth inhibition was assessed with the sulforhodamine B assay in four human tumour cell lines (lung, breast, colon and gastric cancer). The methanolic extract was further characterized in its phenolic composition by HPLC-DAD. The effects of the extract on cell cycle profile and apoptosis were evaluated by flow cytometry and the effect on the expression levels of proteins related to cell cycle and apoptosis was further investigated by Western blotting. Regarding cell growth inhibition, the methanolic extract was the most potent one, particularly in MCF-7 cells (GI50 25.2±0.2lg/ml). Moreover, the GI50 concentration ninduced a G1 cell cycle arrest, with a concomitant decrease in the percentage of cells in the S phase. Furthermore, it caused an increase in the percentage of apoptotic cells, from 6.0±0.2% in untreated cells, to 15.3±2.0% in treated cells. In addition, 48h treatment with the GI50 concentration caused a strong increase in the levels of p53, p21, cleaved caspase-3 and cleaved PARP, together with a decrease in Bcl-2. The main components identified in the methanolic extract were: protocatechuic acid (5.2±0.2mg/kg dw), p-hydroxybenzoic acid (14.1±1.2mg/kg) and cinnamic acid (1.3±0.2mg/kg). Results indicate that Suillus colinitus is a promising source of bioactive compounds. Particularly, its methanolic extract appears to have a p53-mediated effect on the normal cell cycle distribution and apoptosis induction in human breast tumor cells.

* Legal Disclaimer: Chelation and Hyperbaric Therapy, Stem Cell Therapy, and other treatments and modalities mentioned or referred to in this web site are medical techniques that may or may not be considered “mainstream”. As with any medical treatment, results will vary among individuals, and there is no implication or guarantee that you will heal or achieve the same outcome as patients herein.

As with any procedure, there could be pain or other substantial risks involved. These concerns should be discussed with your health care provider prior to any treatment so that you have proper informed consent and understand that there are no guarantees to healing.

THE INFORMATION IN THIS WEBSITE IS OFFERED FOR GENERAL EDUCATIONAL PURPOSES ONLY AND DOES NOT IMPLY OR GIVE MEDICAL ADVICE. No Doctor/Patient relationship shall be deemed to have arisen simply by reading the information contained on these pages, and you should consult with your personal physician/care giver regarding your medical treatment before undergoing any sort of treatment or therapy.

Published on 06-15-2013