Close-up TV News - Prolotheray lecture

Reversing Hypertension

Heavy Metals and all diseases

Close-Up TV News - Dr. Calapai's approach

News 12 Interview: Parkinson’s Disease, Glutathione and Chelation Therapy

News 12 Interview: Platelet-rich plasma therapy

Prolotherapy Interview News 12

News 12 Interview: Diabetes and Weight Loss
Antioxidants reduce cone cell death in a model of retinitis pigmentosa *

In a study involving a rd1 mouse model of retinitis pigmentosa (RP) – a group of diseases which involve damage to the retina, and are caused by a large number of mutations that result in rod photoreceptor cell death, which is followed by a gradual death of cones, the mechanism of which is unknown, characterized by decreased or loss of night vision, peripheral vision, and in advanced stages, central vision – daily injections with various antioxidant supplements (vitamin E, vitamin C, and alpha-lipoic acid) was found to reduce oxidative damage in cones, increase cone cell density, increase medium-wavelength cone opsin mRNA, and preserve some cone function. In this mouse model, by day 18, rods were completely degenerated, and by day 35, 85% of cones were dead. Mice were first injected with a combination of antioxidants, which included alpha-tocopherol (200 mg/kg in olive oil), ascorbic acid (250 mg/kg in PBS), and alpha-lipoic acid (100 mg/kg in PBS/30% ethanol), along with a metalloporphyrin superoxide dismutase mimetic that protects against intracellular generation of reactive oxygen species, MnTBAP (10 mg/kg in PBS). When treated with this combination, some preservation of cone function was found, as determined by significantly greater mean b-wave amplitude found in mice treated with antioxidants, as compared to mice not treated with antioxidants. Furthermore, antioxidants were found to help preserve mRNA synthesis in the cell bodies of one population of cones, as suggested by the significantly greater m-cone opsin mRNA in the retinas of mice treated with antioxidants as compared to those who were not. When the researchers injected the antioxidants individually to determine specific effects of the different antioxidants, it was found that both alpha-tocopherol and alpha-lipoic acid promoted survival of cones after the rods had all died, with approximately 40% of cones surviving, as compared to the other groups. These results suggest that cone cell death which follows rod cell death may be linked to high levels of oxygen in the retina (oxidative damage), and therefore, antioxidant therapy may have a role to play in delaying the progression of vision loss in persons with retinitis pigmentosa. The authors point out the dosages used in this study would not be recommended for humans. Additional research is needed.

* Legal Disclaimer: Chelation and Hyperbaric Therapy, Stem Cell Therapy, and other treatments and modalities mentioned or referred to in this web site are medical techniques that may or may not be considered “mainstream”. As with any medical treatment, results will vary among individuals, and there is no implication or guarantee that you will heal or achieve the same outcome as patients herein.

As with any procedure, there could be pain or other substantial risks involved. These concerns should be discussed with your health care provider prior to any treatment so that you have proper informed consent and understand that there are no guarantees to healing.

THE INFORMATION IN THIS WEBSITE IS OFFERED FOR GENERAL EDUCATIONAL PURPOSES ONLY AND DOES NOT IMPLY OR GIVE MEDICAL ADVICE. No Doctor/Patient relationship shall be deemed to have arisen simply by reading the information contained on these pages, and you should consult with your personal physician/care giver regarding your medical treatment before undergoing any sort of treatment or therapy.

Published on 04-23-2010
Authors: Komeima K, Rogers BS, et al
Source: Proc Natl Acad Sci USA, 2006; 103(20): 11300-5