Close-up TV News - Prolotheray lecture

Reversing Hypertension

Heavy Metals and all diseases

Close-Up TV News - Dr. Calapai's approach

News 12 Interview: Parkinson’s Disease, Glutathione and Chelation Therapy

News 12 Interview: Platelet-rich plasma therapy

Prolotherapy Interview News 12

News 12 Interview: Diabetes and Weight Loss
Anti-oxidative and anti-inflammatory role of adipose stem cells in reversing pulmonary hypertension and associated cardiac remodeling (1090.9) *

Pulmonary Hypertension (PH) is a debilitating cardiopulmonary disorder, often resulting in right heart failure. Existing therapy does not curtail the rate of mortality. Here, the protective role of adipose stem cell (ADSC) in attenuating PH was studied. Monocrotaline (MCT) was injected into 8-weeks old male SD rats to induce PH. Post 2 weeks of MCT challenge, when the RVSP (Right Ventricular Systolic Pressure) was significantly elevated, ADSC were administered. After 4 weeks of MCT induction, RVSP (30.09±1.51 vs 86.71±4.21 mmHg), & RV Hypertrophy (RVH; 0.23±0.01 vs 0.60±0.03) were elevated. ADSC therapy significantly reduced RVSP (55.94±7.35) and RVH (0.34±0.04). Video of echocardiography demonstrated that RV end-diastolic area, (0.12±0.01 vs 0.33±0.03 cm2) and RV ejection fraction (61.62±0.93 vs 29.47±3.76 %) was significantly dysregulated in PH. Further, the pulmonary blood flow (0.88±0.04 vs 0.58±0.06 m/s) and AT/ET (0.37±0.01 vs 0.18±0.02) was altered by MCT treatment. ADSC reverted all of these cardiac remodeling events. RT-PCR demonstrated that the markers of fibrosis (TGFβ), pro-inflammatory cytokines (TLR4, TNFα, IL1β) and oxidative stress (NOX2) were up regulated 4 to 5 fold and anti-inflammatory cytokine (IL10) was down regulated 6 fold in PH animals. ADSC therapy normalized all of these parameters. Collectively, our data demonstrate that the anti-oxidant and anti-inflammatory role of ADSC arrested PH and associated cardiac remodeling and suggest that ADSC intervention could be a potential therapeutic alternative for the treatment of PH.


* Legal Disclaimer: Chelation and Hyperbaric Therapy, Stem Cell Therapy, and other treatments and modalities mentioned or referred to in this web site are medical techniques that may or may not be considered “mainstream”. As with any medical treatment, results will vary among individuals, and there is no implication or guarantee that you will heal or achieve the same outcome as patients herein.

As with any procedure, there could be pain or other substantial risks involved. These concerns should be discussed with your health care provider prior to any treatment so that you have proper informed consent and understand that there are no guarantees to healing.

THE INFORMATION IN THIS WEBSITE IS OFFERED FOR GENERAL EDUCATIONAL PURPOSES ONLY AND DOES NOT IMPLY OR GIVE MEDICAL ADVICE. No Doctor/Patient relationship shall be deemed to have arisen simply by reading the information contained on these pages, and you should consult with your personal physician/care giver regarding your medical treatment before undergoing any sort of treatment or therapy.

Published on 07-11-2014