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Use of a collagen-platelet rich plasma scaffold to stimulate healing of a central defect in the canine ACL Long Island

The anterior cruciate ligament (ACL) of the knee fails to heal after primary repair. Here we hypothesize that a beneficial biologic repair response can be induced by placing a collagen-platelet rich plasma (collagen-PRP) material into a central ACL defect. A collagen-PRP scaffold was used to treat a central ACL defect in vivo. In the first experiment, the histologic response in treated and untreated defects was evaluated at 3 (n = 5) and 6 weeks (n = 5). In the second experiment, biomechanical testing of the treated ligaments (n = 8) was performed at 6 weeks and compared with the results of biomechanical testing of untreated defects at the same time-point (n = 6).

The percentage filling of the defects in the treated ACLs was significantly higher at both the 3- and 6-week time-points when compared with the untreated contralateral control defects (50 ± 21% vs. 2 ± 2% at 3 weeks, and 43 ± 11% vs. 23 ± 11 at 6 weeks; all values mean ± SEM. Biomechanically, the treated ACL defects had a 40% increase in strength at 6 weeks, which was significantly higher than the 14% increase in strength previously reported for untreated defects (p < 0.02). Placement of a collagen-PRP bridging scaffold in a central ACL defect can stimulate healing of the ACL histologically and biomechanically. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 24:820-830, 2006

Published on 05-27-2009
Authors: Martha M. Murray 1 *, Kurt P. Spindler 2, Clint Devin 2, Brian S. Snyder 3, John Muller 3, Masaya Takahashi 3, Percy Ballard 1 2, Lillian B. Nanney 4, David Zurakowski 1
Source: Journal of Orthopaedic Research Volume 24 Issue 4, Pages 820 - 830